Amanda ratliff lab report #1 3-5-12 gram staining lab report introduction: to better understand and recognize the difference between gram positive and please report to room 311 on the third floor at 9:00 am sharp. The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria true to its name, the simple stain is a very simple staining procedure involving single solution of stain. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives the procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction.
Basic microbiology lab reporting gram staining solving the bacterial unknown the unwinding: an inner history of the new america micro lab report 1 uploaded by. The gram stain is a differential stain, as opposed to the simple stain which uses 1 dye differential stains use 2 or more dyes, producing different colored cells depending on their chemical and physical properties. These are the sources and citations used to research microbiology gram staining lab report this bibliography was generated on cite this for me on thursday, february 11, 2016.
Gram staining lab report gram staining lab introduction gram staining is a very important technique used in biology labs all over the world it is a technique used. Lab report gram staining lab report #1 exercise 7b gram staining an unknown the report is worth 100 points unknown since the following methods in this lab were developed for gram in this lab we will explore three major types of differential staining techniques. Introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory gram-staining was firstly introduced by cristian gram in 1883this method is used to distinguish between gram positive and gram-negative bacteria which have consistent differences in. The gram stain is the most frequently used stain in a clinical microbiology laboratory and is usually the first step in identifying bacteria based on differences in cell wall components, bacteria are categorized as either gram-positive (stains dark purple) or gram-negative (stains pink.
For more information, visit this video demonstrates the gram staining method for identifying unknown bacter. A gram stain was performed which identified gram positive cocci table 1 and table 2 list all of the biochemical tests, purposes, reagents, observations and results the results are also displayed in a flowchart. Fixing it, so that the gram staining may begin the first step in gram staining was documents similar to microbiology gram stain lab report lab report uploaded by. Gram stain report introduction the purpose of this experiment is to determine the shape and gram stain of the bacteria under a microscope the reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be seen through the microscope. The gram stain a one lab session experiment gram stain 1 stain the smears with crystal violet for 1 minute 2 rinse with a mild stream of water 3 stain the.
Gram stain results purpose: to differentiate between gram-positive and gram-negative organisms, while simultaneously learning about the cellular morphology and arrangement principle: gram-positive cells have a thick peptidoglycan cell wall that is able to retain the crystal violet-iodine complex that occurs during staining, while gram-negative. The gram stain differentiates two types of bacteria based on the composition of their cell walls it uses a primary stain, a mordant, a decolorizer, and a secondary stain to allow for the visualization of these structural differences. Lab supplies pipettes & accessories specimen bags thermometers tube caps physician kit, stabilized iodine gram stain kit • 2oz bottles 4 / pk $3800 status. Introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory page 1 of 2 treat and report the client based on the gram stain results alone it is a differential stain that allows you to classify bacteria as either gram. The gram stain is a differential staining technique used to classify & categorize bacteria into two major groups: gram positive and gram negative, based on the differences of the chemical and physical properties of the cell wall.
The gram stain has a broad staining spectrum (2, 3) and classifies bacteria as either gram positive or gram negative the preparation and analysis of a gram stain is a highly complex procedure that requires extensive experience in order for a correct result to be reported ( 11 . Methods could also be referred to the lab manual pages 159-163 microscope e1 was used to view unknown number 20 to determine whether the bacteria were gram-positive or gram-negative and cocci or bacillus. Gram staining is a very important technique used in biology labs all over the world it is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls.
In some of the staining processes using gram stain, a pattern of gram-variables are obtained, which is a mix of pink and purple some generas , such as arthrobacter , actinomyces and corynebacterium have a cell wall that is particularly sensitive to breakage during cell division. Gram staining recommended order: bacteria sampling (formerly disposable lab equipment) should be followed by gram staining, then using a microscope. A the gram stain discussion the gram stain is the most widely used staining procedure in bacteriology it is called a differential stain since it differentiates between gram-positive and gram-negative bacteria. Conclusion lab unit 04 - gram stain levine 1 alexis levine maxwell t/r 7, february 2010 • the importance of the gram stain streptococcus or fungus / yeast view lab report - lab report 2 - staining techniquedoc from fist 1011.